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Evaluation of boronate-containing polymer brushes and gels as substrates for carbohydrate-mediated adhesion and cultivation of animal cells.

På svenska

Author

  • Alexander Ivanov
  • Ashok Kumar
  • Suthasinee Nilsang
  • Maria-Rosa Aguilar
  • Lyubov I Mikhalovska
  • Irina N Savina
  • Lars Nilsson
  • Ivan Scheblykin
  • Marina Kuzimenkova
  • Igor Galaev

Summary, in English

Boronate-containing thin polyacrylamide gels (B-Gel), polymer brushes (B-Brush) and chemisorbed organosilane layers (B-COSL) were prepared on the surface of glass slides and studied as substrates for carbohydrate-mediated cell adhesion. B-COSL- and B-Brush-modified glass samples exhibited multiple submicron structures densely and irregularly distributed on the glass surface, as found by scanning electron microscopy and atomic force microscopy. B-Gel was ca. 0.1mm thick and contained pores with effective size of 1-2mum in the middle and of 5-20mum on the edges of the gel sample as found by confocal laser scanning microscopy. Evidence for the presence of phenylboronic acid in the samples was given by time-of-flight secondary ion mass-spectrometry (ToF SIMS), contact angle measurements performed in the presence of fructose, and staining with Alizarin Red S dye capable of formation specific, fluorescent complexes with boronic acids. A comparative study of adhesion and cultivation of animal cells on the above substrates was carried out using murine hybridoma M2139 cell line as a model. M2139 cells adhered to the substrates in the culture medium without glucose or sodium pyruvate at pH 8.0, and then were cultivated in the same medium at pH 7.2 for 4 days. It was found that the substrates of B-Brush type were superior both regarding cell adhesion and viability of the adhered cells, among the substrates studied. MTT assay confirmed proliferation of M2139 cells on B-Brush substrates. Some cell adhesion was also registered in the macropores of B-Gel substrate. The effects of surface microstructure of the boronate-containing polymers on cell adhesion are discussed. Transparent glass substrates grafted with boronate-containing copolymers offer good prospects for cell adhesion studies and development of cell-based assays.

Department/s

Publishing year

2010

Language

English

Pages

510-519

Publication/Series

Colloids and Surfaces. B, Biointerfaces

Volume

75

Document type

Journal article

Publisher

Elsevier

Topic

  • Physical Chemistry

Status

Published

ISBN/ISSN/Other

  • ISSN: 1873-4367