Sperm DNA integrity in relation to exposure to environmental perfluoroalkyl substances - A study of spouses of pregnant women in three geographical regions

Summary, in English

Perfluoroalkyl substances (PFASs) can interfere with male reproductive function, but evidence in humans is limited. Six hundred four fertile men (199 from Greenland, 197 from Poland and 208 from Ukraine) were enrolled in the study. We measured four PFASs in serum (PFOS, PFOA, PFNA and PFHxS) and concurrent DNA damage in spermatozoa by sperm chromatin structure assay (SCSA) and in situ terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay, apoptotic markers in semen (Fas-receptor and Bcl-xL), and reproductive hormones in serum. No association between PFASs and SCSA, apoptotic markers or reproductive hormones emerged. We observed a slight increase in SHBG and TUNEL-positivity with increased PFOA exposure in men from Greenland. Thus, consistent evidence that PFAS exposure interferes with sperm DNA fragmentation, apoptosis or reproductive hormones was not found. (C) 2012 Elsevier Inc. All rights reserved.

• Author: Ina Olmer Specht; Karin S. Hougaard; Marcello Spano; Davide Bizzaro; Gian Carlo Manicardi; Christian Lindh; Gunnar Toft; Bo A Jönsson; Aleksander Giwercman; Jens Peter E. Bonde
• Department/s: Division of Occupational and Environmental Medicine, Lund University; Reproductive medicine, Malmö
• Publishing year: 2012
• Language: English
• Pages: 577-583
• Publication/Series: Reproductive Toxicology
• Volume: 33
• Issue: 4
• Links: http://dx.doi.org/10.1016/j.reprotox.2012.02.008
• Document type: Journal article
• Publisher: Elsevier
• Topic: Obstetrics, Gynecology and Reproductive Medicine
• Keywords: Perfluorinated compounds; PFOS; PFOA; DNA fragmentation index; Sperm; chromatin structure assay; TUNEL assay; Fas receptor; Bcl-xL
• Status: Published
• Research group: Reproductive medicine, Malmö
• ISBN/ISSN/Other: ISSN: 1873-1708