An adaptive alignment algorithm for quality-controlled label-free LC-MS.
Author
Summary, in English
Label-free quantification using precursor-based intensities is a versatile workflow for large-scale proteomics studies. The method requires extensive computational analysis and is therefore in need of robust quality control during the data mining stage. We present a new label-free data analysis workflow integrated into a multi-user software platform. A novel adaptive alignment algorithm has been developed to minimize the possible systematic bias introduced into the analysis. Parameters are estimated on the fly from the data at hand, producing a user-friendly analysis suite. Quality metrics are output in every step of the analysis as well as actively incorporated into the parameter estimation. We furthermore show the improvement of this system by comprehensive comparison to classical label-free analysis methodology as well as current state-of-the-art software.
Department/s
Publishing year
2013
Language
English
Pages
1407-1420
Publication/Series
Molecular & Cellular Proteomics
Volume
12
Issue
5
Links
Document type
Journal article
Publisher
American Society for Biochemistry and Molecular Biology
Topic
- Endocrinology and Diabetes
Status
Published
Research group
- Tornblad Institute
ISBN/ISSN/Other
- ISSN: 1535-9484