Stability of peptide-HLA-I complexes and tapasin folding facilitation - tools to define immunogenic peptides
Author
Summary, in English
Only a small fraction of the peptides generated inside the cell end up being presented by HLA-I on the cell surface. High stability of peptide-HLA-I complexes and a low HLA-I tapasin-facilitation have been proposed to predict immunogenicity. We here set out to investigate if these parameters correlated and defined immunogenic peptides. Both peptide-HLA-B*08:01 and peptide-HLA-A*02:01 complexes showed small differences in tapasin-facilitation and larger differences in stability. This suggests that the stability of immunogenic peptide-HLA-I complexes vary above an HLA-I allomorph dependent lower limit (e. g. > 2 h for HLA-A*02:01), immunogenicity predicted by tapasin-facilitation may be defined by an equally allomorph unique upper value (e. g. tapasin-facilitation <1.5 for HLA-A*02:01), and variation above the stability-threshold does not directly reflect a variation in tapasin-facilitation. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
Department/s
Publishing year
2012
Language
English
Pages
1336-1343
Publication/Series
FEBS Letters
Volume
586
Issue
9
Links
Document type
Journal article
Publisher
Wiley-Blackwell
Topic
- Biological Sciences
Keywords
- Tapasin
- MHC-I
- Peptide
- Stability
- Vaccine
Status
Published
Research group
- Antigen Presentation
ISBN/ISSN/Other
- ISSN: 1873-3468