Monoclonal anti-mouse laminin antibodies: AL-1 reacts with laminin alpha1 chain, AL-2 with laminin beta1 chain, and AL-4 with the coiled-coil domain of laminin beta1 chain.

Summary, in English

We analyzed the reactivity of three different commercially available rat monoclonal antibodies raised against mouse laminin-a1 beta 1 gamma 1 (laminin-11), AL-1, AL-2, and AL-4. Using ELISA assays, Western blot analysis and inummostainings we present refined epitope maps for these three laminin monoclonals. AL-1 reacted, as predicted with laminin alpha 1 chain. AL-4 has also been marketed as an alpha 1 chain specific probe, but we show here that AL-4 detects mouse laminin beta 1 chain, in the distal part of the coiled-coil region. AL-2 was predicted to react with all three chains near the cross-region, but seems to primarily react with laminin beta 1 chain. (c) 2006 Elsevier B.V./Intemational Society of Matrix Biology. All rights reserved.

• Author: Susanne Schéele; Takako Sasaki; Anna Arnal-Estapé; Madeleine Durbeej-Hjalt; Peter Ekblom
• Department/s: Faculty of Medicine; Muscle Biology; Department of Experimental Medical Science
• Publishing year: 2006
• Language: English
• Pages: 301-305
• Publication/Series: Matrix Biology
• Volume: 25
• Issue: 5
• Full text: - 340 kB; Download statistics
• Links: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16631359&dopt=Abstract; http://dx.doi.org/10.1016/j.matbio.2006.03.004
• Document type: Journal article
• Publisher: Elsevier
• Topic: Basic Medicine; Cancer and Oncology
• Keywords: kidney; muscle; recombinant proteins; monoclonal antibodies; laminin; ELISA
• Status: Published
• Research group: Muscle Biology
• ISBN/ISSN/Other: ISSN: 1569-1802