The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here: https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

In vitro sliding of actin filaments labelled with single quantum dots

På svenska

Author

Summary, in English

We recently refined the in vitro motility assay for studies of actomyosin function to achieve rectified myosin induced sliding of actin filaments. This paves the way, both for detailed functional studies of actomyosin and for nanotechnological applications. In the latter applications it would be desirable to use actin filaments for transportation of cargoes (e.g., enzymes) between different predetermined locations on a chip. We here describe how single quantum dot labelling of isolated actin filaments simultaneously provides handles for cargo attachment and bright and photostable fluorescence labels facilitating cargo detection and filament tracking. Labelling was achieved with preserved actomyosin function using streptavidin-coated CdSe quantum dots (Qdots). These nanocrystals have several unique physical properties and the present work describes their first use for functional studies of isolated proteins outside the cell. The results, in addition to the nanotechnology developments, open for new types of in vitro assays of isolated biomolecules. (C) 2003 Elsevier Inc. All rights reserved.

Publishing year

2004

Language

English

Pages

529-534

Publication/Series

Biochemical and Biophysical Research Communications

Volume

314

Issue

2

Document type

Journal article

Publisher

Elsevier

Topic

  • Biological Sciences

Keywords

  • nanocrystal
  • nanostructure
  • quantum dot
  • myosin
  • actin
  • motility assay

Status

Published

ISBN/ISSN/Other

  • ISSN: 1090-2104