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Importance of obtaining independent measures of insulin secretion and insulin sensitivity during the same test - Results with the Botnia clamp

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Author

  • Devjit Tripathy
  • Y Wessman
  • M Gullstrom
  • T Tuomi
  • Leif Groop

Summary, in English

OBJECTIVE - To validate and apply a method for independent assessment of insulin secretion and insulin sensitivity (S-l) during file same test that is, an intravenous glucose tolerance test followed by a euglycemic-hyperinsulinemic clamp, also called the Botnia clamp. This test was then applied to nondiabetic subjects with (FH+)and without (FH-) a first-degree family history of diabetes. RESEARCH DESIGN AND METHODS - The Botnia clamp measures the first-phase insulin response (FPIR) to 0.3g/k, glucose i.v. and insulin sensitivity (NI-value) front a 2-h euglycemic clamp begun 60 ruin alter the glucose bolus. The W-value obtained during the Botnia clamp was compared With M-values obtained during a regular euglycemic clamp without prior glucose bolus. Repeated tests were performed in random order in subjects with normal and abnormal glucose tolerance, Finally, the test was applied to subjects with and without a family history of type 2 diabetes. RESULTS - S-l and insulin secretion from this test showed a high degree of reproducibility, and the M-value obtained with the Botnia clamp correlated strongly,with the M-value from a euglycemic clamp without prior glucose bolus (r - 0.953, P < 0.005). FH+ subjects showed decreased S-l (P = 0.02), hut similar FPIR, compared with FH- subjects, However, insulin Secretion adjusted for file degree of insulin resistance was significantly impaired (P = 0.04). CONCLUSIONS - In conclusion, the Botnia clamp provides reliable and independent measures of S-l and beta-cell function during, the same test. As illustrated above, knowledge of the degree of S-l is mandatory When presenting data on insulin secretion.

Publishing year

2003

Language

English

Pages

1395-1401

Publication/Series

Diabetes Care

Volume

26

Issue

5

Document type

Journal article

Publisher

American Diabetes Association

Topic

  • Endocrinology and Diabetes

Status

Published

Research group

  • Translational Muscle Research

ISBN/ISSN/Other

  • ISSN: 1935-5548