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Composition and structure of mixed phospholipid supported bilayers formed by POPC and DPPC

På svenska

Author

  • Anna Akesson
  • Tania Lind
  • Nicky Ehrlich
  • Dimitrios Stamou
  • Hanna Wacklin
  • Marite Cardenas

Summary, in English

In this paper we present a systematic study of the morphology and composition of supported lipid bilayers (SLBs) formed by vesicle fusion using a wide variety of surface sensitive techniques that give information about the lateral as well as vertical structure and bilayer fluidity. SLBs of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) mixtures at five different bulk vesicle compositions were formed in such a way that the phase separation boundaries were crossed. For all compositions studied, the SLBs were systematically enriched with POPC compared to the nominal vesicle composition. Nevertheless, gel-fluid domain coexistence was observed for SLB compositions in which phase separation was expected based on the bulk phase diagram. The probable causes for the compositional difference in the SLBs are discussed in terms of the phase behaviour of the mixture and its effect on the membrane formation process by vesicle fusion.

Publishing year

2012

Language

English

Pages

5658-5665

Publication/Series

Soft Matter

Volume

8

Issue

20

Document type

Journal article

Publisher

Royal Society of Chemistry

Topic

  • Natural Sciences
  • Physical Sciences

Status

Published

ISBN/ISSN/Other

  • ISSN: 1744-6848