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Cytoskeleton components of inside-out and right-side-out plasma membrane vesicles from plants

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Author

Summary, in English

Isolated plasma membrane vesicles purified by aqueous polymer two-phase partitioning were used as a model system for studies on the membrane-associated (cortical) cytoskeleton in plants. Actin, as identified by immunoblotting, was found to be specifically attached to plasma membrane vesicles from cauliflower (Brassica oleracea L.). The actin was not washed off as the vesicles were turned inside-out, indicative of a fairly strong attachment. Triton X-100 extraction of plasma membrane vesicles resulted in an insoluble and hence pelletable fraction where actin could be found together with several other proteins. Our results show that the cortical cytoskeleton is to some extent co-purified with the plasma membrane, and we believe that well defined, inside-out and right-side-out plasma membrane vesicles can be used to study the structure and dynamics of the plant cortical cytoskeleton.

Publishing year

1993

Language

English

Pages

45-52

Publication/Series

Protoplasma

Volume

177

Issue

1-2

Document type

Journal article

Publisher

Springer

Topic

  • Biological Sciences

Keywords

  • Actin
  • Cauliflower
  • Cortical cytoskeleton
  • Membrane cytoskeleton
  • Plant plasma membrane.

Status

Published

ISBN/ISSN/Other

  • ISSN: 1615-6102