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Photopatterning of self assembled monolayers on oxide surfaces for the selective attachment of biomolecules

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Author

  • Pompi Hazarika
  • Jonathan M. Behrendt
  • Linn Petersson
  • Christer Wingren
  • Michael L. Turner

Summary, in English

The immobilization of functional biomolecules to surfaces is a critical process for the development of biosensors for disease diagnostics. In this work we report the patterned attachment of single chain fragment variable (scFv) antibodies to the surface of metal oxides by the photodeprotection of self-assembled monolayers, using near-UV light. The photodeprotection step alters the functionality at the surface; revealing amino groups that are utilized to bind biomolecules in the exposed regions of the substrate only. The patterned antibodies are used for the detection of specific disease biomarker proteins in buffer and in complex samples such as human serum. (C) 2013 Elsevier B.V. All rights reserved.

Publishing year

2014

Language

English

Pages

82-89

Publication/Series

Biosensors & Bioelectronics

Volume

53

Document type

Journal article

Publisher

Elsevier

Topic

  • Immunology in the medical area

Keywords

  • Self-assembled monolayer (SAM)
  • Silicon
  • Photo-pattern
  • Proteins
  • Biomarker detection

Status

Published

ISBN/ISSN/Other

  • ISSN: 1873-4235