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Peptides analysis in blood plasma using on-line system of supported liquid membrane and high-performance liquid chromatography

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Author

Summary, in English

The potential of using supported liquid membrane (SLM) technique, combined with reversed-phase high-performance liquid chromatography (RP-HPLC) has been investigated for the determination of peptides in human blood plasma. The peptides studied were (DL)Leu(DL)Phe, MetLeuPhe, GlyLeuTyr and ValGluProlleProTyr. The carrier (Aliquat 336) was incorporated in membrane phase in order to facilitate the transport of investigated peptides. After extraction, the analyte-enriched acceptor phase was directly injected into an HPLC system for analysis. With SLM, high selectivity and efficiency were achieved for extraction of peptides in aqueous solutions. Lower extraction efficiency was obtained in plasma sample. The limiting factors were the presence of salts and proteins in blood plasma. The necessary of ultrafiltration application before the SLM was required in order to improve its stability. The minimum quantifiable concentration was 90-130 ng/ml of the investigated peptides based on 0.5 ml plasma. The standard curve was linear over the concentration range of 0.05-10 mu g/ml. (c) 2005 Elsevier B.V. All rights reserved.

Publishing year

2005

Language

English

Pages

9-14

Publication/Series

Analytica Chimica Acta

Volume

553

Issue

1-2

Document type

Journal article

Publisher

Elsevier

Topic

  • Analytical Chemistry

Status

Published

ISBN/ISSN/Other

  • ISSN: 1873-4324