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Real-time Fluorescence Imaging of Capillary Electrophoresis

På svenska

Author

  • S Nilsson
  • Jonas Johansson
  • M Mecklenburg
  • S Birnbaum
  • Sune Svanberg
  • K. G Wahlund
  • K Mosbach
  • A Miyabayashi
  • P. O Larsson

Summary, in English

An arrangement for real-time fluorescence imaging of capillary electrophoretic separations is described. A decoated capillary was excited along a substantial part of its length by a dye laser pumped with an XeCl excimer laser tuned to match the fluorophors in the sample. Light emitted from the migrating, fluorescent molecules was detected by an image-intensified, thermoelectrically cooled charge-coupled device camera. The camera signals were processed by a computer and displayed as moving peaks on a screen in real-time, thus allowing the progress of the separation to be monitored continuously. Basic characteristics of the imaging arrangement such as sensitivity (signal-to-noise ratio) and resolution were checked with fluorescein samples. The imaging principle was also illustrated with a separation of a mixture of DNA fragments on a commercially available capillary designated for DNA separations. The benefits of real-time imaging for separation processes, in particular for capillary electrophoresis, are discussed.

Department/s

Publishing year

1995

Language

English

Pages

46-52

Publication/Series

Journal of Capillary Electrophoresis

Volume

2

Issue

1

Document type

Journal article

Publisher

International Scientific Communications Inc.

Topic

  • Atom and Molecular Physics and Optics

Status

Published

ISBN/ISSN/Other

  • ISSN: 1079-5383