The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here: https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

Determination of hexahydrophthalic and methylhexahydrophthalic acids in urine by gas chromatography-negative-ion chemical-ionisation mass spectrometry

På svenska

Author

Summary, in English

A method for determining hexahydrophthalic (HHP acid) and methylhexahydrophthalic acids (MHHP acid) from human urine was developed. These acids are metabolites of the highly sensitising hexahydrophthalic anhydride and methylhexahydrophthalic anhydride. The acids were purified from urine by liquid-solid extraction and derivatised with pentafluorobenzyl bromide to the corresponding esters. These were analysed by GC-MS in the negative-ion chemical-ionisation mode with ammonia moderating gas. Deuterium labelled HHP acid and MHHP acid were internal standards. The precision for HHP acid was 3–6% in the range 70–620 ng mL–1 and that for MHHP acid was 2–8% in the range 60–680 ng mL–1. Overall recovery of HHP acid from urine was 74–88% and that for MHHP acid 98–101%. Detection limits were 11 ng HHP acid mL–1 urine and 17 ng MHHP acid mL–1 urine. There were no significant differences between determinations of HHP acid by a previous method and the present method. Some instability of the acids in urine were found after extended storage at –20°C. The method was applicable for determination of HHP acid and MHHP acid from exposed workers.

Publishing year

1996

Language

English

Pages

647-652

Publication/Series

Chromatographia

Volume

42

Issue

11-12

Document type

Journal article

Publisher

Vieweg Verlag

Topic

  • Environmental Health and Occupational Health

Keywords

  • Industrial hygiene
  • Biological monitoring
  • Pentafluorobenzyl derivatives
  • Hexahydrophthalic and methylhexahydrophthalic acid
  • Gas chromatography-mass spectrometry

Status

Published

ISBN/ISSN/Other

  • ISSN: 0009-5893