Proteomics in Nasal Lavage Fluids from Persulfate Challenged Hairdressers
Author
Summary, in English
A high throughput screening method for quantification of 246 NLF proteins was developed using selected reaction monitoring (SRM). Also, a strategy for identification of multiple oxidative modifications was developed and applied on proteins oxidized in vitro by persulfate. SRM assays targeting the oxidized peptides were developed. NLF samples from a previous persulfate challenge study were analyzed. Hairdressers with bleaching powder associated rhinitis were compared to hairdressers without respiratory symptoms and with atopic subjects without work related exposure to persulfate. Protein abundances in the NLF samples were analyzed with two different proteomic approaches. Pooled samples were first analyzed with an untargeted approach using 2D-nanoLC-MS/MS combined with iTRAQ-labeling and then with the SRM method targeting 246 NLF proteins. Also, oxidized peptides were measured in the samples by SRM.
Several proteins with biologically relevant functions were changed after the persulfate challenge. The exact role of these proteins in relation to persulfate-induced respiratory symptoms needs to be further studied as well as the usefulness of them as effect biomarkers. Persulfates oxidized tryptophan and methionine residues. It was concluded that oxidized peptides containing these modifications are not suitable as persulfate exposure biomarkers due to poor specificity. Peptides containing oxidized tryptophan may be useful as biomarkers for oxidative stress and should be further evaluated.
Publishing year
2014
Language
English
Publication/Series
Lund University Faculty of Medicine Doctoral Dissertation Series
Volume
2014:30
Full text
Document type
Dissertation
Publisher
Division of Occupational and Environmental Medicine
Topic
- Environmental Health and Occupational Health
Keywords
- nasal lavage fluid
- selected reaction monitoring
- persulfate
- hairdresser
- proteomics
- oxidation
- mass spectrometry
- iTRAQ
Status
Published
Supervisor
- Christian Lindh
- Bo A Jönsson
- Monica Kåredal
ISBN/ISSN/Other
- ISSN: 1652-8220
- ISBN: 978-91-87651-54-0
Defence date
21 March 2014
Defence time
09:15
Defence place
Belfragesalen, BMC D15, Klinikgatan 32, Lund
Opponent
- Jonas Bergquist (Professor)