Synthesis of dipeptide units of the D D configuration in aqueous media by enzymatic catalysis
Author
Summary, in English
Novel molecularly imprinted polymer systems utilizing 4-vinylpyridine and 1-vinylimidazole as functional monomers have been developed for enantioselective recognition of carboxylic and N-protected amino acids. Non-covalent interactions between the functional monomers and the template molecules were the source of the subsequent recognition sites in the resultant polymers. The capacity of the polymers for molecular recognition was investigated by using them as stationary phases in the HPLC mode. Polymers prepared with 4-vinylpyridine were found to be more efficient in racemic resolution than those prepared with 1-vinylimidazole. When applying a racemic mixture of the template molecule, the polymers showed highest affinity for the enantiomer used as template. Imprints of a racemic template molecule, as expected, did not exhibit enantioselectivity. The optimal molar ratio of 4-vinylpyridine to the template Cbz-L-Asp-OH in the polymerization mixture was determined to be 12:1. In addition to enantioselectivity, the investigated polymers demonstrated ligand selectivity e.g., a Cbz-L-Asp-OH-imprinted polymer was able to separate Cbz-D,L-Asp-OH, but was unable to separate Cbz-D,L-Glu-OH.
Publishing year
1993
Language
English
Pages
25-29
Publication/Series
Journal of Molecular Recognition
Volume
6
Issue
1
Document type
Journal article
Publisher
John Wiley & Sons Inc.
Topic
- Biochemistry and Molecular Biology
Status
Published
Research group
- Nanomedicine and Biomaterials
ISBN/ISSN/Other
- ISSN: 1099-1352