Hereditary Nonpolyposis Colorectal Cancer - Molecular Genetics and Biology of Associated Tumors
Author
Summary, in English
In paper I, we screened 16 families with suspected HNPCC for germline MMR gene mutations and found a diverse spectrum of mutations, involving the MMR genes <i>MLH1, MSH2</i> and <i>MSH6</i>. A defective MMR is associated with microsatellite instability (MSI) in the tumor tissue and with somatic mutations in repeated sequences in several cancer-associated genes. In paper II, we studied the occurrence of such alterations in 24 tumors from 14 individuals in an HNPCC family with a germline <i>MSH2</i> mutation and found an extensive intra- and inter-individual variation. Paper III demonstrates intratumoral heterogeneity of repeat-mutations in 10 macroscopically different areas of a colon carcinoma in a patient with a germline <i>MLH1</i> mutation. The variation in the somatic mutations in repeat-containing genes suggests that these alterations are important for tumor progression rather than initiation and that the accumulation of mutations, rather than the specific alterations, drives HNPCC tumorigenesis.
MMR defects play a role also in the development of sporadic (non-hereditary) cancer and are found in about 15% of colon cancers. In paper IV, we investigated rectal cancer patients regarding a family history of cancer and MSI in the tumor tissue. Only 3/165 (2%) of the tumors had MSI and all 3 patients were found to carry germline HNPCC-causing mutations. We conclude that MSI is rare in rectal cancer, but, when present, strongly indicates HNPCC. In paper V, we studied MSI and immunohistochemical expression of the MMR proteins in small bowel adenocarcinomas and found MSI in 11/70 (16%) tumors, 7 of which showed loss of MMR expression. Defective MMR thus contributes to small bowel carcinogenesis in a fraction of the tumors similar to colon cancer. In paper VI, we studied a population-based series of women who developed the two most common cancer types in HNPCC, colorectal cancer and endometrial cancer, before age 50. MSI was demonstrated in 75% of the tumors and concordant loss of the same MMR protein in both tumors, suggesting an underlying MMR gene mutation, was found in 12/27 patients.
In summary, this thesis presents novel HNPCC-causing mutations, demonstrates variability among somatic mutations in repeat-containing genes in HNPCC-tumors, delineates the contribution of defective MMR in rectal cancer and small bowel cancer and points to a high risk of HNPCC among women with colorectal and endometrial cancer at young age.
Department/s
Publishing year
2002
Language
English
Document type
Dissertation
Publisher
Maria Planck, Department of Oncology, Lund University Hospital, SE-221 85 Lund, Sweden,
Topic
- Cancer and Oncology
Keywords
- endometrial cancer
- rectal cancer
- colon cancer
- mismatch repair protein expression
- coding repeats
- microsatellite instability
- MSH6
- MSH2
- Hereditary nonpolyposis colorectal cancer
- MLH1
- small bowel cancer
- Cytology
- oncology
- cancerology
- Cytologi
- onkologi
- cancer
Status
Published
Supervisor
- [unknown] [unknown]
ISBN/ISSN/Other
- ISBN: 91-628-5146-2
Defence date
5 April 2002
Defence time
09:00
Defence place
The Lecture Hall, Department of Oncology, Lund University Hospital
Opponent
- Annika Lindblom (Professor)