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Miniaturization of multiplexed planar recombinant antibody arrays for serum protein profiling.

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Author

  • Linn Petersson
  • Michael Coen
  • Nabil A Amro
  • Lennart Truedsson
  • Carl Ak Borrebaeck
  • Christer Wingren

Summary, in English

Background: Antibody-based microarrays are a developing tool for high-throughput proteomics in health and disease. However, in order to enable global proteome profiling, novel miniaturized high-density antibody array formats must be developed. Results: In this proof-of-concept study, we have designed a miniaturized planar recombinant (single-chain Fragment variable). antibody array technology platform for multiplexed profiling of non-fractionated, directly labelled serum samples. The size of the individual spot features was reduced 225-times (78.5 μm(2)/spot) and the array density was increased 19-times (38,000 spots/cm(2)). These miniaturized, multiplexed arrays were produced, using a desktop nanofabrication system based on dip-pen nanolithography technology, and interfaced with a high-resolution fluorescent-based scanner. The reproducibility, sensitivity, specificity, and applicability of the set-up were demonstrated by profiling a set of well-characterized serum samples. Conclusion: The designed antibody array platform opens up new possibilities for large-scale, multiplex profiling of crude proteomes in a miniaturized fashion.

Publishing year

2014

Language

English

Pages

1175-1185

Publication/Series

Bioanalysis

Volume

6

Issue

9

Document type

Journal article

Publisher

Future Science

Topic

  • Pharmacology and Toxicology

Status

Published

ISBN/ISSN/Other

  • ISSN: 1757-6180