GUTI: a new antigen in the Cromer blood group system

Summary, in English

BACKGROUND: The Cromer blood group system consists of seven high-incidence and three low-incidence antigens carried on decay-accelerating factor (DAF). This report describes the identification and characterization of a new Cromer high-incidence antigen, named GUTI. STUDY DESIGN AND METHODS: RT-PCR and sequence analysis were performed on cDNA prepared from a Chilean donor whose serum contained the alloantibody (anti-GUTI). Based on the observed point mutation, a PCR-RFLP assay using MaeII was developed. To map the epitope, DAF-deletion mutants were tested by immunoblotting with anti-GUTI. RESULTS: Sequence analysis revealed a substitution of 719G>A in DAF in the proband. The proband's parents and two daughters were heterozygotes for 719G>A, one sister whose RBCs typed GUTI- was homozygous for 719A, and one sister had the wild-type DAF (719G). Seven additional heterozygote samples were identified among 214 Chileans. No heterozygotes were found in 197 New York donors. Analysis using DAF-deletion mutants showed the antigenic determinant to be within short consensus repeat (SCR) 4. CONCLUSION: This study describes a novel high- incidence antigen (GUTI) in the Cromer blood group system characterized by the amino acid arginine at position 206 in SCR4 of DAF. The GUTI-negative proband has a substitution mutation that predicts for histidine at this position.

• Author: Jill Storry; Laima Sausais; Kim Hue-Roye; Flora Mudiwa; Zennie Ferrer; Morris A Blajchman; Douglas M Lublin; Bei-Wen Ma; Juan F Miquel; Flavio Nervi; Jaime Pereira; Marion E Reid
• Publishing year: 2003
• Language: English
• Pages: 340-344
• Publication/Series: Transfusion
• Volume: 43
• Issue: 3
• Links: http://dx.doi.org/10.1046/j.1537-2995.2003.00319.x
• Document type: Journal article
• Publisher: Wiley-Blackwell
• Topic: Hematology
• Status: Published
• ISBN/ISSN/Other: ISSN: 1537-2995