Development of Duffy transgenic mouse: in vivo expression of human Duffy gene with -33T -> C promoter mutation in non-erythroid tissues
Author
Summary, in English
Blood group Duffy gene (FY) promoter in Duffy-negative individuals contains a point mutation in the GATA1 protein-binding motif, which was suggested to be responsible for erythroid suppression of FY. We developed two transgenic mouse lines with FY from both Duffy phenotypes. Transgenic mice with FY from Duffy-positive phenotype expressed Duffy protein both in red blood cells (RBCs) and non-erythroid tissues. Transgenic mice with FY from Duffy-negative phenotype did not express Duffy protein in RBCs, but it was expressed in non-erythroid tissues. This is the first in vivo experimental evidence showing the effect of -33T-->C promoter mutation on FY expression.
Department/s
Publishing year
2004
Language
English
Pages
356-359
Publication/Series
British Journal of Haematology
Volume
127
Issue
3
Links
Document type
Journal article
Publisher
Wiley-Blackwell
Topic
- Hematology
Keywords
- erythroid-non-erythroid expression
- transgenic mice
- Duffy gene (FY)
- -33T -> C mutation
Status
Published
ISBN/ISSN/Other
- ISSN: 0007-1048