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A pTalpha-negative subpopulation of CD25+ TN thymocytes revealed by a transgenic marker.

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Author

Summary, in English

We have recently generated 5'lambda5-huTAC mice, which express the human CD25 (huTAC) gene under the control of the 5'-flanking region of the mouse lambda5-gene. The huTAC-transgene was expressed in pre-B cells but neither in mature B cells nor in T cells of these mice. In this report we demonstrate that the transgene is also transiently expressed by adult CD25+ CD3-CD4-CD8- (triple negative, TN) thymocytes and in fetal thymocytes. The huTAC+, in contrast to the huTAC- subpopulation of the CD44+CD25+ TN cells, was unexpectedly found not to express the pTalpha-gene. Still the huTAC+CD44+CD25+ TN cells reconstituted the development of both alphabeta and gammadelta lineage cells equally efficiently as the pTalpha-expressing huTAC- fraction, demonstrating that this pTalpha-negative subpopulation contained precursors for both T-cell lineages. Single cell reverse transcription-polymerase chain reaction (RT-PCR) experiments demonstrated that also in normal mice only a fraction of CD44+CD25+ and CD44-CD25+ TN cells expressed this gene. Taken together, these data indicate that huTAC transgene expression revealed a truly pTalpha-negative fraction of the CD44+CD25+ TN cells. The observation that not all precursors in the CD25+ TN population express the pTalpha-gene has important implications for the understanding of early T-cell development and T-cell lineage commitment.

Department/s

  • Immunology

Publishing year

2002

Language

English

Pages

119-128

Publication/Series

Scandinavian Journal of Immunology

Volume

55

Issue

2

Document type

Journal article

Publisher

Wiley-Blackwell

Topic

  • Immunology in the medical area

Keywords

  • Antigen
  • T-Cell
  • gamma-delta/metabolism
  • Receptors
  • Interleukin-2/*genetics/*metabolism
  • alpha-beta/metabolism
  • Transgenic
  • Mice
  • Membrane Glycoproteins/*genetics
  • Liver/cytology/immunology
  • Hematopoietic Stem Cells/immunology
  • Genetic Markers
  • Reporter
  • Genes
  • Gene Expression
  • Fetus/cytology/immunology
  • B-Lymphocytes/immunology
  • CD44/metabolism
  • Animal
  • Antigens
  • T-Lymphocyte Subsets/*immunology
  • Thymus Gland/cytology/immunology

Status

Published

Research group

  • Immunology

ISBN/ISSN/Other

  • ISSN: 1365-3083