Comparison of (GTG)(5)-oligonucleotide and ribosomal intergenic transcribed spacer (ITS)-PCR for molecular typing of Klebsiella isolates

Summary, in English

Molecular typing of Klebsiella species has become important for monitoring dissemination of beta-lactamase-producers in hospital environments. The present study was designed to evaluate poly-trinucleotide (GTG)(5)- and rDNA intergenic transcribed spacer (ITS)-PCR fingerprint analysis for typing of Klebsiella pneumoniae and Klebsiella oxytoca isolates. Multiple displacement amplified DNA derived from 19K pneumoniae (some with an ESBL-phenotype). 35 K. oxytoca isolates, five K pneumoniae, two K oxytoca, three Raoultella, and one Enterobacter aerogenes type and reference strains underwent (GTG)(5) and ITS-PCR analysis. Dendrograms were constructed using cosine coefficient and the Neighbour joining method. (GTG)(5) and ITS-PCR analysis revealed that K pneumoniae and K oxytoca isolates. reference and type strains formed distinct cluster groups, and tentative subclusters could be established. We conclude that (GTG)(5) and ITS-PCR analysis combined with automated capillary electrophoresis provides promising tools for molecular typing of Klebsiella isolates. (C) 2010 Elsevier B.V. All rights reserved.

• Author: Anna Ryberg; Crister Olsson; Siv Ahrné; Hans-Juerg Monstein
• Department/s: Surgery; Division of Food and Pharma
• Publishing year: 2011
• Language: English
• Pages: 183-188
• Publication/Series: Journal of Microbiological Methods
• Volume: 84
• Issue: 2
• Links: http://dx.doi.org/10.1016/j.mimet.2010.11.019
• Document type: Journal article
• Publisher: Elsevier
• Topic: Surgery
• Keywords: Klebsiella spp.; Molecular typing; (GTG)(5)-PCR; Ribosomal intergenic; transcribed spacer (ITS)-PCR; Fingerprint analysis; Multiple; displacement amplification
• Status: Published
• Research group: Surgery
• ISBN/ISSN/Other: ISSN: 1872-8359