Saccharomyces cerevisiae RAP1 bind to telomeric sequences with spatial flexibility
Author
Summary, in English
A wide divergence has been detected in the telomeric sequences among budding yeast species. Despite their length and homogeneity differences, all these yeast telomeric sequences show a conserved core which closely matches the consensus RAP1-binding sequence. We demonstrate that the RAP1 protein binds this sequence core, without involving the diverged sequences outside the core. in Saccharomyces castellii and Saccharomyces dairensis specific classes of interspersed variant repeats are present. We show here that a RAP1-binding site is formed in these species by connecting two consecutive 8 bp telomeric repeats. DNase I footprint analyses specify the binding site as the 13 bp sequence CTGGGT-GTCTGGG, The RAP1 protein also binds the variant repeats, although with a lowered affinity. However, a split footprint is produced when RAP1 binds a variant repeat where the two half-sites of the binding site are separated by an additional 6 nt. This is probably caused by the intervening sequence looping out of the RAP1-DNA complex. We suggest that the bipartite subdomain structure of the RAP1 protein allows it to remodel telomeric chromatin, a feature which may be of great relevance for telomeric chromatin assembly and structure in vivo.
Department/s
- Department of Biology
- Molecular Genetics and Genetics
Publishing year
2000
Language
English
Pages
2292-2301
Publication/Series
Nucleic Acids Research
Volume
28
Issue
12
Document type
Journal article
Publisher
Oxford University Press
Topic
- Genetics
Status
Published
Research group
- Molecular Genetics and Genetics
ISBN/ISSN/Other
- ISSN: 1362-4962