Dispersion of viable pig liver cells with collagenase
Author
Summary, in English
Viable suspended hepatocytes were prepared from surgical biopsy specimens of pig and human liver by digestion with collagenase. Initial perfusion of the tissue through cannulated blood vessels with 0.5 mM EGTA followed by 0.2% collagenase gave the best results. 20−870 × 106 cells of which 60–95 % excluded trypan blue were obtained from 5–30 g pig liver pieces, while results with human liver specimens were usually less satisfactory. In some experiments, however, viable cells, as judged by vital stain exclusion and ability to synthesize lipids were obtained in sufficient yield. In the pig hepatocytes glycerolipid synthesis from [3H] glycerol and oxidation and esterification of [14C] oleic acid had the same characteristics as those observed earlier in rat hepatocytes.
Department/s
Publishing year
1975
Language
English
Pages
1219-1225
Publication/Series
Life Sciences
Volume
17
Issue
8
Document type
Journal article
Publisher
Elsevier
Topic
- Other Clinical Medicine
- Cancer and Oncology
- Surgery
- Pediatrics
- Cell and Molecular Biology
Status
Published
ISBN/ISSN/Other
- ISSN: 1879-0631