The Complement Inhibitor CD59 Regulates Insulin Secretion by Modulating Exocytotic Events.
Author
Summary, in English
Type 2 diabetes is triggered by reduced insulin production, caused by genetic and environmental factors such as inflammation originating from the innate immune system. Complement proteins are a component of innate immunity and kill non-self cells by perforating the plasma membrane, a reaction prevented by CD59. Human pancreatic islets express CD59 at very high levels. CD59 is primarily known as a plasma membrane protein in membrane rafts, but most CD59 protein in pancreatic β cells is intracellular. Removing extracellular CD59 disrupts membrane rafts and moderately stimulates insulin secretion, whereas silencing intracellular CD59 markedly suppresses regulated secretion by exocytosis, as demonstrated by TIRF imaging. CD59 interacts with the exocytotic proteins VAMP2 and Syntaxin-1. CD59 expression is reduced by glucose and in rodent diabetes models but upregulated in human diabetic islets, potentially reflecting compensatory reactions. This unconventional action of CD59 broadens the established view of innate immunity in type 2 diabetes.
Department/s
Publishing year
2014
Language
English
Pages
883-890
Publication/Series
Cell Metabolism
Volume
19
Issue
5
Full text
Links
Document type
Journal article
Publisher
Cell Press
Topic
- Cell and Molecular Biology
Status
Published
Research group
- Diabetes - Islet Patophysiology
- Protein Chemistry, Malmö
- Diabetic Complications
- Genomics, Diabetes and Endocrinology
- Celiac Disease and Diabetes Unit
- Islet cell physiology
ISBN/ISSN/Other
- ISSN: 1550-4131