Determination of N-methylsuccinimide and 2-hydroxy-N-methylsuccinimide in human urine and plasma

Summary, in English

A method for determination of N-methylsuccinimide (MSI) and 2-hydroxy-N-methylsuccinimide (2-HMSI) in human urine and of MSI in human plasma was developed. MSI and 2-HMSI are metabolites of the widely used organic solvent N-methyl-2-pyrrolidone (NMP). MSI and 2-HMSI were purified from urine and plasma by C8 solid-phase extraction and analysed by gas chromatography-mass spectrometry in the negative-ion chemical ionisation mode. The intra-day precisions in urine were 2-6% for MSI (50 and 400 ng/ml) and 3-5% for 2-HMSI (1000 and 8000 ng/ml). For MSI in plasma it was 2% (60 and 1200 ng/ml). The between-day precisions in urine were 3-4% for MSI (100 and 1000 ng/ml) and 2-4% for 2-HMSI (10,000 and 18,000 ng/ml) and 3-4% for MSI in plasma (100 and 900 ng/ml). The recoveries from urine were 109-117% for MSI (50 and 400 ng/ml) and 81-89% for 2-HMSI (1000 and 8000 ng/ml). The recovery of MSI from plasma was 91-101% (50 and 500 ng/ml). The detection limits for MSI were 3 ng/ml in urine and 1 ng/ml in plasma and that of 2-HMSI in urine was 200 ng/ml. The method is applicable for analysis of urine and plasma samples from workers exposed to NMP.

• Author: Bo A Jönsson; Bengt Åkesson
• Department/s: Division of Occupational and Environmental Medicine, Lund University
• Publishing year: 1997
• Language: English
• Pages: 151-158
• Publication/Series: Journal of Chromatography. B
• Volume: 704
• Issue: 1-2
• Links: http://dx.doi.org/10.1016/S0378-4347(97)00452-0
• Document type: Journal article
• Publisher: Elsevier
• Topic: Environmental Health and Occupational Health
• Keywords: N-Methylsuccinimide; 2-Hydroxy-N-methylsuccinimide; N-Methyl-2-pyrrolidone
• Status: Published
• ISBN/ISSN/Other: ISSN: 1387-2273