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Automated solid-phase extraction and liquid chromatography tandem mass spectrometry determination of N-methyl-2-pyrrolidone and its metabolites in urine and plasma

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Author

  • Arieh Cohen
  • Bo A Jönsson

Summary, in English

A method for the simultaneous determination of N-methyl-2-pyrrolidone (NMP) and its metabolites 5-hydroxyl-N-pyrrolidone (5HNMP), N-methylsuccinimide (MSI) and 2-hydroxy-N-methylsuccinimide (2HMSI) in plasma and urine has been developed. Samples were purified by SPE using an ASPEC XL4. Analysis was performed using LC-MS equipped with an APCI interface. The analysis provided linear responses in the range of 0.125-12 mu g mL(-1) for all of the analytes and up to 150 mu g mL(-1) for 5HNMP and 2HMS1. The within day precision was in the range of 0.9-19.1% for plasma samples and 1.9-10.4% for urine samples whereas the between day precisions were 4.5-11.9% and 1.2-17.5%, respectively. The method was deemed to be suitable for monitoring the levels of NMP and its metabolites in the plasma and urine of occupationally exposed persons.

Publishing year

2007

Language

English

Pages

407-412

Publication/Series

Chromatographia

Volume

65

Issue

7-8

Document type

Journal article

Publisher

Vieweg Verlag

Topic

  • Environmental Health and Occupational Health

Keywords

  • column liquid chromatography-mass spectrometry
  • solid-phase extraction
  • N-methyl-2-pyrrolidone in Urine and plasma

Status

Published

ISBN/ISSN/Other

  • ISSN: 0009-5893