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TNF-alpha Sensitizes HT-29 Colonic Epithelial Cells to Intestinal Lactobacilli.

På svenska

Author

  • Vance J McCracken
  • Taehoon Chun
  • Manuel E Baldeón
  • Siv Ahrné
  • Göran Molin
  • Roderick I Mackie
  • H Rex Gaskins

Summary, in English

The ability of the proinflammatory cytokine tumor necrosis factor-alpha (TNF-alpha) to influence epithelial interleukin (IL)-8 responses to the intestinal bacterium Lactobacillus plantarum 299v was analyzed in the human HT-29 colonic epithelial cell line. In the absence of TNF-alpha, IL-8 mRNA expression was not detectable by Northern blot analysis in HT-29 cells alone or in HT-29 cells co-cultured with L. plantarum 299v. However, TNF-alpha induced IL-8 mRNA expression, and co-culture of TNF-alpha-treated HT-29 cells with L. plantarum 299v significantly increased IL-8 mRNA expression above levels induced by TNF-alpha alone in an adhesion-dependent manner. The increase in IL-8 mRNA expression was not observed in TNF-alpha-treated HT-29/L. plantarum 299v co-cultures using heat-killed lactobacilli or when L. plantarum adhesion was prevented using mannoside or a trans-well membrane. Paradoxically, IL-8 secretion was decreased in TNF-alpha-treated HT-29 cells with L. plantarum 299v relative to cells treated with TNF-alpha alone. TNF-alpha-mediated responsiveness to L. plantarum 299v was further investigated by analyzing expression of a coreceptor for bacterial cell wall products CD14. HT-29 cells expressed CD14 mRNA and cell-surface CD14; however, TNF-alpha did not alter CD14 mRNA or cell-surface expression, and blockade of CD14 with monoclonal antibody MY4 did not alter the IL-8 response to L. plantarum 299v in TNF-alpha-treated HT-29 cells. These results indicate that although TNF-alpha sensitizes HT-29 epithelial cells to intestinal lactobacilli, the bacteria exert a protective effect by downregulating IL-8 secretion.

Department/s

  • Department of Food Technology, Engineering and Nutrition

Publishing year

2002

Language

English

Pages

665-670

Publication/Series

Experimental Biology and Medicine

Volume

227

Issue

8

Document type

Journal article

Publisher

SAGE Publications

Topic

  • Food Engineering

Keywords

  • Lactobacillus
  • intestinal epithelial cell
  • interleukin-8
  • CD14

Status

Published

ISBN/ISSN/Other

  • ISSN: 1535-3702