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Polymerase chain reaction for detection of Mycobacterium tuberculosis

På svenska

Author

  • Ulf Sjöbring
  • Michael Mecklenburg
  • Åse Bengård Andersen
  • Håkan Miörner

Summary, in English

A polymerase chain reaction for the specific detection of mycobacteria belonging to the Mycobacterium tuberculosis complex was developed. Using a single primer pair derived from the nucleotide sequence of protein antigen b of M. tuberculosis, we achieved specific amplification of a 419-base-pair DNA fragment in M. tuberculosis and M. bovis. After DNA was extracted from mycobacteria by using a simple, safe lysis procedure, we detected the 419-base-pair sequence in samples containing few mycobacteria. Preliminary data suggested that this technique could be applied to clinical specimens for early and specific diagnosis of tuberculosis.

Publishing year

1990

Language

English

Pages

2200-2204

Publication/Series

Journal of Clinical Microbiology

Volume

28

Issue

10

Document type

Journal article

Publisher

American Society for Microbiology

Topic

  • Microbiology in the medical area

Status

Published

ISBN/ISSN/Other

  • ISSN: 1098-660X