Actin-like protein associated with plasma membranes from Euglena gracilis
Author
Summary, in English
Microtubules are characteristic components of the membrane skeleton of Euglena gracilis, but whether microfilaments are present has been controversial. We here present evidence that an actin-like protein may indeed be associated with the plasma membrane (PM) of E. gracilis. Firstly, a 47 kDa, PM-associated, polypeptide was recognized by an anti-amoeba actin antibody. Secondly, this 47 kDa protein seemed to be peripherally attached to PM in much the same way as β-tubulin, since both could be released from PM by treatment with 150 mM NaOH but not with ethylene glycol, NaCl, or formamide. Thirdly, the 47 kDa polypeptide and β-tubulin were found mainly in the Triton X-1 14-insoluble fraction, indicating that they were part of a protein complex resistant to detergents, such as the cytoskeleton. Finally, DNase I activity was inhibited by a fraction enriched in the 47 kDa polypeptide, a property typical of actin.
Department/s
Publishing year
1998
Language
English
Pages
153-160
Publication/Series
Protoplasma
Volume
202
Issue
3-4
Document type
Journal article
Publisher
Springer
Topic
- Biological Sciences
Keywords
- Actin
- Anti-actin antibody
- Cytoskeleton
- Euglena gracilis
- Triton X-114
- DNAse I
Status
Published
ISBN/ISSN/Other
- ISSN: 1615-6102