A fast semi-quantitative LC-MS method for measurement of intact apolipoprotein A-I reveals novel proteoforms in serum.

Summary, in English

Surrogate markers for reverse cholesterol transport (RCT) efficiency such as HDL cholesterol and immune methods for apolipoprotein A-I (ApoA-I) may not fully reflect the actual efficiency of the RCT pathway. Several genetic variants and different posttranslational proteoforms of ApoA-I may unevenly affect the functionality of the HDL particle to efflux cholesterol. A method employing top-down immunoaffinity LC-MS of ApoA-I in order to characterize the most prevalent ApoA-I proteoforms in human plasma is described.

• Author: Mats Gåfvels; Per Bengtson
• Department/s: Division of Clinical Chemistry and Pharmacology
• Publishing year: 2015
• Language: English
• Pages: 87-95
• Publication/Series: Clinica Chimica Acta
• Volume: 442
• Issue: Jan 17
• Links: http://www.ncbi.nlm.nih.gov/pubmed/25603406?dopt=Abstract; http://dx.doi.org/10.1016/j.cca.2015.01.011
• Document type: Journal article
• Publisher: Elsevier
• Topic: Clinical Laboratory Medicine
• Status: Published
• ISBN/ISSN/Other: ISSN: 0009-8981