The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here: https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

Efficient sample preparation in immuno-matrix-assisted laser desorption/ionization mass spectrometry using acoustic trapping

På svenska

Author

Summary, in English

Acoustic trapping of minute bead amounts against fluid flow allows for easy automation of multiple assay steps, using a convenient aspirate/dispense format. Here, a method based on acoustic trapping that allows sample preparation for immuno-matrix-assisted laser desorption/ionization mass spectrometry using only half a million 2.8 mu m antibody covered beads is presented. The acoustic trapping is done in 200 x 2000 mu m2 glass capillaries and provides highly efficient binding and washing conditions, as shown by complete removal of detergents and sample processing times of 5-10 min. The versatility of the method is demonstrated using an antibody against Angiotensin I (Ang I), a peptide hormone involved in hypotension. Using this model system, the acoustic trapping was efficient in enriching Angiotensin at 400 pM spiked in plasma samples. (C) 2013 American Institute of Physics. [http://dx.doi.org/10.1063/1.4798473]

Publishing year

2013

Language

English

Publication/Series

Biomicrofluidics

Volume

7

Issue

2

Document type

Journal article

Publisher

American Institute of Physics (AIP)

Topic

  • Medical Engineering

Keywords

  • acoustic applications
  • biological specimen preparation
  • molecular
  • biophysics
  • organic compounds
  • time of flight mass spectroscopy

Status

Published

ISBN/ISSN/Other

  • ISSN: 1932-1058