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Antisense repression of the mitochondrial nadh-binding subunit of complex I in transgenic potato plants affects male fertility

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Author

Summary, in English

Mitochondrial respiratory chain complex I is a large multi-subunit enzyme composed of both organellar and nuclear encoded proteins. To investigate the role of the nuclear encoded components, expression of the gene for the 55 kDa NADH-binding subunit of complex I was disturbed by antisense repression in transgenic potato plants. The antisense construct driven by the CaMV 35S promoter decreases the steady-state mRNA levels of transcripts for the 55 kDa subunit to 33% of the wild type levels. Quantities of the 55 kDa protein in mitochondrial protein extracts are lowered to about 50% in these plants. Transgenic plants show normal vegetative growth and tuber formation, but pollen maturation is found to be disturbed. The reduced male fertility of the transgenic 55 kDa antisense plants may be caused by an insufficient mitochondrial respiratory chain, impaired by the decreased expression of the NADH-binding component of mitochondrial complex I.

Publishing year

1997-08-27

Language

English

Pages

61-69

Publication/Series

Plant Science

Volume

127

Issue

1

Document type

Journal article

Publisher

Elsevier

Topic

  • Biochemistry and Molecular Biology

Keywords

  • Antisense repression
  • Male fertility
  • Mitochondrial NADH-dehydrogenase (complex I)
  • Solanum tuberosum

Status

Published

Research group

  • Plant Biology

ISBN/ISSN/Other

  • ISSN: 0168-9452