A folded and immunogenic IgE-hyporeactive variant of the major allergen Phl p 1 produced in Escherichia coli.

Summary, in English

Group 1 grass pollen allergens are a major cause of allergic disease. Specific immunotherapy involving controlled administration of allergens can be used as a disease-modifying treatment for such disease. Recombinant allergen variants with reduced IgE binding capacity may be used as component in such vaccines, as they may induce fewer treatment side effects than materials currently in use. A mutated variant of the immunodominant C-terminal domain of the group 1 grass pollen allergen Phl p 1 was recently established through an approach that used a set of human monoclonal IgE as a guide to identify mutations that disturbed IgE-allergen interactions. Further analysis of this domain is required to establish its potential for use in treatment.

• Author: Mattias Levin; Harm Otten; Claes von Wachenfeldt; Mats Ohlin
• Department/s: Department of Immunotechnology; MAX IV Laboratory; Molecular Cell Biology; Microbiology Group
• Publishing year: 2015
• Language: English
• Publication/Series: BMC Biotechnology
• Volume: 15
• Links: http://dx.doi.org/10.1186/s12896-015-0150-z
• Document type: Journal article
• Publisher: BioMed Central (BMC)
• Topic: Respiratory Medicine and Allergy
• Status: Published
• Project: Human IgE repertoires and an anti-allergome resource
• Research group: Microbiology Group
• ISBN/ISSN/Other: ISSN: 1472-6750