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Stable Homogeneous Gel for Molecular-Sieving of DNA Fragments in Capillary Electrophoresis

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Author

  • Ákos Végvári
  • Stellan Hjertén

Summary, in English

A polyacrylamide gel crosslinked with allyl-b-cyclodextrin can be used repeatedly for several weeks for the separation of DNA fragments, since bubbles are not generated during a run. Allyl-b-cyclodextrin can easily be synthesized in one step from allylglycidylether and b-cyclodextrin. The plate numbers for DNA fragments, up to about 1500 bp, are high: for the 21 separation of pBR322/HaeIII fragments they were in the range 450 000–1 600 000 m . The resolution was almost independent of the concentration of the crosslinker (allyl-b-cyclodextrin) — in sharp contrast to gels crosslinked with N,N9-methylenebisacrylamide.

Publishing year

2002

Language

English

Pages

221-227

Publication/Series

Journal of Chromatography A

Volume

960

Issue

1-2

Document type

Journal article

Publisher

Elsevier

Topic

  • Medical Engineering

Keywords

  • Capillary electrophoresis
  • DNA
  • Polyacrylamide
  • Allyl-b-cyclodextrin

Status

Published

ISBN/ISSN/Other

  • ISSN: 0021-9673