Integrated acoustic immunoaffinity-capture (IAI) platform for detection of PSA from whole blood samples.
Author
Summary, in English
On-chip detection of low abundant protein biomarkers is of interest to enable point-of-care diagnostics. Using a simple form of integration, we have realized an integrated microfluidic platform for the detection of prostate specific antigen (PSA), directly in anti-coagulated whole blood. We combine acoustophoresis-based separation of plasma from undiluted whole blood with a miniaturized immunoassay system in a polymer manifold, demonstrating improved assay speed on our Integrated Acoustic Immunoaffinity-capture (IAI) platform. The IAI platform separates plasma from undiluted whole blood by means of acoustophoresis and provides cell free plasma of clinical quality at a rate of 10 uL/min for an online immunoaffinity-capture of PSA on a porous silicon antibody microarray. The whole blood input (hematocrit 38-40%) rate was 50 μl min(-1) giving a plasma volume fraction yield of ≈33%. PSA was immunoaffinity-captured directly from spiked female whole blood samples at clinically significant levels of 1.7-100 ng ml(-1) within 15 min and was subsequently detected via fluorescence readout, showing a linear response over the entire range with a coefficient of variation of 13%.
Department/s
- EpiHealth: Epidemiology for Health
- BioCARE: Biomarkers in Cancer Medicine improving Health Care, Education and Innovation
- Department of Biomedical Engineering
- Department of Laboratory Medicine
- Clinical Chemistry, Malmö
Publishing year
2013
Language
English
Pages
1790-1796
Publication/Series
Lab on a Chip
Volume
13
Issue
9
Full text
- Available as PDF - 716 kB
- Download statistics
Links
Document type
Journal article
Publisher
Royal Society of Chemistry
Topic
- Clinical Medicine
- Medicinal Chemistry
Status
Published
Research group
- Clinical Chemistry, Malmö
ISBN/ISSN/Other
- ISSN: 1473-0189