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Improvements in the order, isotropy and electron density of glypican-1 crystals by controlled dehydration.

På svenska

Author

  • Wael Awad
  • Gabriel Svensson Birkedal
  • Marjolein M G M Thunnissen
  • Katrin Mani
  • Derek T Logan

Summary, in English

The use of controlled dehydration for improvement of protein crystal diffraction quality is increasing in popularity, although there are still relatively few documented examples of success. A study has been carried out to establish whether controlled dehydration could be used to improve the anisotropy of crystals of the core protein of the human proteoglycan glypican-1. Crystals were subjected to controlled dehydration using the HC1 device. The optimal protocol for dehydration was developed by careful investigation of the following parameters: dehydration rate, final relative humidity and total incubation time Tinc. Of these, the most important was shown to be Tinc. After dehydration using the optimal protocol the crystals showed significantly reduced anisotropy and improved electron density, allowing the building of previously disordered parts of the structure.

Publishing year

2013

Language

English

Pages

2524-2533

Publication/Series

Acta Crystallographica. Section D: Biological Crystallography

Volume

69

Issue

Pt 12

Document type

Journal article

Publisher

John Wiley & Sons Inc.

Topic

  • Structural Biology

Status

Published

Research group

  • Glycobiology

ISBN/ISSN/Other

  • ISSN: 1399-0047