Comparison of the proximal promoter regions of the PAX3 and PAX7 genes
Author
Summary, in English
Translocations t(2;13)(q35;q14) and t(1;13)(p36;q14), which fuse PAX3 and PAX7, respectively, to FOXO1A, characterize alveolar rhabdomyosarcoma. Previous studies have suggested that the expression of PAX7-FOXO1A is copy-number dependent, but that of PAX3-FOXO1A is not, which may be due to a weaker PAX7 than PAX3 promoter. The aim of the present study was to compare the transcriptional activities of the PAX3 and PAX7 proximal promoter regions, using the dual-luciferase reporter assay with three vector systems in eight cell lines. The PAX3 promoter was found to have higher transcriptional activity than that of PAX7 irrespective of the vector system or cell line used. These findings are consistent with the idea that an amplification event is required for the PAX7-FOXO1A chimeric transcript to reach a critical expression level.
Department/s
Publishing year
2007-10-15
Language
English
Pages
114-119
Publication/Series
Cancer Genetics and Cytogenetics
Volume
178
Issue
2
Links
Document type
Journal article
Publisher
Elsevier
Topic
- Medical Genetics
- Cancer and Oncology
Keywords
- 3T3 Cells
- Animals
- Cell Line
- Child
- Chromosome Aberrations
- DNA Primers
- Gene Amplification
- Genes, Reporter
- HeLa Cells
- Humans
- Mice
- PAX7 Transcription Factor
- Paired Box Transcription Factors
- Plasmids
- Promoter Regions, Genetic
- Reverse Transcriptase Polymerase Chain Reaction
- Rhabdomyosarcoma
- Transcription, Genetic
- Transfection
- Translocation, Genetic
- Journal Article
- Research Support, Non-U.S. Gov't
Status
Published
ISBN/ISSN/Other
- ISSN: 0165-4608