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Orthogonal scaffold of magnetically aligned collagen lamellae for corneal stroma reconstruction

På svenska

Author

  • Jim Torbet
  • Marilyne Malbouyres
  • Nicolas Builles
  • Virginie Justin
  • Muriel Roulet
  • Odile Damour
  • Åke Oldberg
  • Florence Ruggieo
  • David J. S. Hulmes

Summary, in English

The creation of 3D scaffolds that mimic the structure of physiological tissue required for normal cell function is a major bioengineering challenge. For corneal stroma reconstruction this necessitates the creation of a stroma-like scaffold consisting of a stack of orthogonally disposed sheets of aligned collagen fibrils. This study demonstrates that such a scaffold can be built up using magnetic alignment. By allowing neutralized acid-soluble type I collagen to gel in a horizontal magnetic field (7 T) and by combining a series of gelation-rotation-gelation cycles, a scaffold of orthogonal lamellac composed of aligned collagen fibrils has been formed. Although initially dilute, the gels can be concentrated without noticeable loss in orientation. The gels are translucent but their transparency can be greatly improved by the addition of proteoglycans to the gel-forming solution. Keratocytes align by contact guidance along the direction of collagen fibrils and respect the orthogonal design of the collagen template as they penetrate into the bulk of the 3D matrix. The scaffold is a significant step towards the creation of a corneal substitute with properties resembling those of native corneal stroma. (c) 2007 Elsevier Ltd. All rights reserved.

Department/s

  • Åke Oldberg´s group

Publishing year

2007

Language

English

Pages

4268-4276

Publication/Series

Biomaterials

Volume

28

Issue

29

Document type

Journal article

Publisher

Elsevier

Topic

  • Bioengineering Equipment

Keywords

  • stroma-like scaffold
  • magnetic alignment
  • keratocytes
  • proteoglycans
  • corneal stroma reconstruction
  • collagen

Status

Published

Research group

  • Åke Oldberg´s group

ISBN/ISSN/Other

  • ISSN: 1878-5905