The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here: https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

Deep sequencing extends the diversity of human papillomaviruses in human skin.

På svenska

Author

  • Davit Bzhalava
  • Laila Sara Arroyo Mühr
  • Camilla Lagheden
  • Johanna Ekström
  • Ola Forslund
  • Joakim Dillner
  • Emilie Hultin

Summary, in English

Most viruses in human skin are known to be human papillomaviruses (HPVs). Previous sequencing of skin samples has identified 273 different cutaneous HPV types, including 47 previously unknown types. In the present study, we wished to extend prior studies using deeper sequencing. This deeper sequencing without prior PCR of a pool of 142 whole genome amplified skin lesions identified 23 known HPV types, 3 novel putative HPV types and 4 non-HPV viruses. The complete sequence was obtained for one of the known putative types and almost the complete sequence was obtained for one of the novel putative types. In addition, sequencing of amplimers from HPV consensus PCR of 326 skin lesions detected 385 different HPV types, including 226 previously unknown putative types. In conclusion, metagenomic deep sequencing of human skin samples identified no less than 396 different HPV types in human skin, out of which 229 putative HPV types were previously unknown.

Publishing year

2014

Language

English

Publication/Series

Scientific Reports

Volume

4

Issue

Jul 24

Document type

Journal article

Publisher

Nature Publishing Group

Topic

  • Microbiology in the medical area
  • Other Clinical Medicine

Status

Published

Research group

  • Clinical Microbiology, Malmö
  • Internal Medicine - Epidemiology

ISBN/ISSN/Other

  • ISSN: 2045-2322