A β-mannan utilisation locus in Bacteroides ovatus involves a GH36 α-galactosidase active on galactomannans
Author
Summary, in English
The Bacova_02091 gene in the β-mannan utilisation locus of Bacteroides ovatus encodes a family GH36 α-galactosidase (BoGal36A), transcriptionally upregulated during growth on galactomannan. Characterisation of recombinant BoGal36A reveals unique properties compared to other GH36 α-galactosidases, which preferentially hydrolyse terminal α-galactose in raffinose family oligosaccharides. BoGal36A prefers hydrolysing internal galactose substitutions from intact and depolymerized galactomannan. BoGal36A efficiently releases (>90%) galactose from guar and locust bean galactomannans, resulting in precipitation of the polysaccharides. As compared to other GH36 structures, the BoGal36A 3D model displays a loop deletion, resulting in a wider active site cleft which likely can accommodate a galactose-substituted polymannose backbone. This article is protected by copyright. All rights reserved.
Department/s
Publishing year
2016-06-11
Language
English
Pages
2106-2118
Publication/Series
FEBS Letters
Volume
590
Issue
14
Document type
Journal article
Publisher
Wiley-Blackwell
Topic
- Structural Biology
- Biochemistry and Molecular Biology
Status
Published
ISBN/ISSN/Other
- ISSN: 1873-3468