Supercritical Fluid Extraction and Chromatography of Lipids in Bilberry.
Author
Summary, in English
A supercritical fluid extraction (SFE) method has been developed for the extraction of lipids in bilberry. Experimental design was used to optimize pressure, temperature and extraction time using CO2 as solvent. Best SFE condition for total lipids was 450 bar, 60 °C and 45 min. The SFE method was compared to conventional Bligh & Dyer (B&D) extraction. The amount of fatty acid methyl esters (FAME) was found to be 4.84 ± 0.06 mg and 4.564 ± 0.003 mg per g of the freeze-dried bilberry sample for the developed SFE and B&D methods, respectively, while the amount of total lipids was found to be 54.40 ± 6.06 mg and 65.70 ± 0.67 mg per g of sample for SFE and B&D, respectively. This discrepancy between FAME and total lipids could be explained by the presence of wax esters, sterol esters, carotenoids and phospholipids, as determined by supercritical fluid chromatography.
Department/s
Publishing year
2015
Language
English
Pages
1103-1111
Publication/Series
Journal of the American Oil Chemists Society
Volume
92
Issue
8
Document type
Journal article
Publisher
The American Oil Chemists' Society
Topic
- Industrial Biotechnology
Status
Published
ISBN/ISSN/Other
- ISSN: 0003-021X