Pasteurella multocida thymidine kinase 1 efficiently activates pyrimidine nucleoside analogs.
Author
Summary, in English
In the Pasteurella multocida genome only one putative deoxyribonucleoside kinase encoding gene, for thymidine kinase 1 (PmTK1), was identified. The PmTK1 gene was sub-cloned into Escherichia coli KY895 and it sensitized the host towards 2',2'-difluoro-deoxycytidine (gemcitabine, dFdC), 3'-azido-thymidine (AZT) and 5-fluoro-deoxyuridine (5F-dU). PmTK1 was over-expressed and purified with two different tags. Apparently, deoxyuridine (dU), and not thymidine (dT), is the preferred substrate. We suggest that PmTK1s could be employed as a species-specific activator of uracil-based nucleoside antibiotics.
Department/s
Publishing year
2010
Language
English
Pages
359-362
Publication/Series
Nucleosides, Nucleotides & Nucleic Acids
Volume
29
Issue
4-6
Document type
Journal article
Publisher
Taylor & Francis
Topic
- Biological Sciences
Status
Published
ISBN/ISSN/Other
- ISSN: 1525-7770