Improved matrix-assisted laser desorption/ionisation sample preparation of a partially depolymerised cellulose derivative by continuous spray deposition and interfacing with size-exclusion chromatography
Author
Summary, in English
Continuous spray deposition (CSD) of aqueous solutions of partially depolymerised methyl cellulose was found to improve matrix-assisted laser desorption/ionisation (MALDI) sample preparation. One feature was that the sensitivity in MALDI time-of-flight mass spectrometry increased up to an order of magnitude compared with the standard sample preparation method. Another feature was that CSD provided targets for MALDI with homogeneously distributed analyte. This resulted in a more even signal intensity and a higher reproducibility than in the standard method. High-mass discrimination was more pronounced in CSD than in the standard method. Size-exclusion chromatography with aqueous eluent was coupled online to CSD onto matrix-precoated foils. The suitability for determination of the molar mass distribution of methyl cellulose was investigated. Copyright (c) 2005 John Wiley C Sons, Ltd.
Department/s
Publishing year
2005
Language
English
Pages
947-954
Publication/Series
Rapid Communications in Mass Spectrometry
Volume
19
Issue
7
Document type
Journal article
Publisher
John Wiley & Sons Inc.
Topic
- Analytical Chemistry
Status
Published
ISBN/ISSN/Other
- ISSN: 1097-0231