The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here: https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

Mycobacteria-infected bystander macrophages trigger maturation of dendritic cells and enhance their ability to mediate HIV transinfection

På svenska

Author

  • Jolanta Mazurek
  • Lech Ignatowicz
  • Gunilla Kallenius
  • Marianne Jansson
  • Andrzej Pawlowski

Summary, in English

Synergistic interplay between Mycobacterium tuberculosis (Mtb) and HIV in coinfected ind-ividuals leads to the acceleration of both tuberculosis and HIVdisease. Mtb, as well as HIV, may modulate the function of many immune cells, including DCs. To dissect the bystander impact of Mfs infected with Mtb on DC functionality, we here investigated changes in DC phenotype, cytokine profiles, and HIV-1 transinfecting ability. An in vitro system was used in which human monocyte-derived DCs were exposed to soluble factors released by Mfs infected with mycobacteria, including virulent clinical Mtb isolates and nonvirulent BCG. Soluble factors secreted from Mtb-infected Mfs, and to a lesser extent BCG-infected Mfs, resulted in the production of proinflammatory cytokines and partial upregulation of DC maturation markers. Interestingly, the HIV-1 transinfecting ability of DCs was enhanced upon exposure to soluble factors released by Mtb-infected Mfs. In summary, our study shows that DCs exposed to soluble factors released by mycobacteria-infected Mfs undergo maturation and display an augmented ability to transmit HIV-1 in trans. These findings highlight the important role of bystander effects during the course of MtbHIV coinfection and suggest that Mtb-infected Mfs may contribute to an environment that supports DC-mediated spread and amplification of HIV in coinfected individuals.

Publishing year

2012

Language

English

Pages

1192-1202

Publication/Series

European Journal of Immunology

Volume

42

Issue

5

Document type

Journal article

Publisher

John Wiley & Sons Inc.

Topic

  • Immunology in the medical area

Keywords

  • coinfection center dot dendritic cell center dot HIV-1 center dot
  • macrophage center dot Mycobacterium tuberculosis

Status

Published

Research group

  • HIV-1 and HIV-2 host interactions

ISBN/ISSN/Other

  • ISSN: 1521-4141