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Phylogenetically diverse TT virus viremia among pregnant women

På svenska

Author

  • Davit Bzhalava
  • Johanna Ekström
  • Fredrik Lysholm
  • Emilie Hultin
  • Helena Faust
  • Bengt Persson
  • Matti Lehtinen
  • Ethel-Michele de Villiers
  • Joakim Dillner

Summary, in English

Infections during pregnancy have been suggested to be involved in childhood leukemias. We used high-throughput sequencing to describe the viruses most readily detectable in serum samples of pregnant women. Serum DNA of 112 mothers to leukemic children was amplified using whole genome amplification. Sequencing identified one TT virus (TTV) isolate belonging to a known type and two putatively new TTVs. For 22 mothers, we also performed ITV amplification by general primer PCR before sequencing. This detected 39 TTVs, two of which were identical to the Tilts found after whole genome amplification. Altogether, we found 40 TTV isolates, 29 of which were putatively new types (similarities ranging from 89% to 69%). In conclusion, high throughput sequencing is useful to describe the known or unknown viruses that are present in serum samples of pregnant women. (C) 2012 Elsevier Inc. All rights reserved.

Publishing year

2012

Language

English

Pages

427-434

Publication/Series

Virology

Volume

432

Issue

2

Document type

Journal article

Publisher

Elsevier

Topic

  • Microbiology in the medical area

Keywords

  • Childhood leukemia
  • TT virus
  • High throughput sequencing

Status

Published

Research group

  • Clinical Microbiology, Malmö

ISBN/ISSN/Other

  • ISSN: 1096-0341