Mechanisms of adaptation to the effects of ethanol on activation of phospholipase C in NG 108-15 cells

Summary, in English

In this study the effect of different times of exposure to ethanol (1-7 days, 100 mM) on bradykinin and GTP(S)-stimulated activation of phospholipase C in NG 108-15 cells and on the binding of [3H]bradykinin to its receptors was investigated. Ethanol attenuated both agonist and GTP-analogue-induced hydrolysis of phosphoinositides for a period of up to 4 days of treatment, while exerting no effect on binding to bradykinin receptors. However, after 7 days of exposure to ethanol, the agonist-induced activation of phospholipase C was completely resistant to the inhibitory effects of alcohol. This finding correlated to a change in the affinity of the bradykinin receptor population after 7 days of treatment. The results indicate that bradykinin-induced breakdown of phosphatidylinositol 4,5-bisphosphate adapts to the effects of ethanol, after long-term treatment. Possible adaptative changes taking place at the level of the G protein(s), may induce a shift in the affinity of the receptor population and, consequently, serve as a compensatory mechanism to counteract the inhibitory effect of ethanol.

• Author: F D Rodriguez; Per Simonsson; L Gustavsson; Christer Alling
• Department/s: Clinical Chemistry, Malmö; Division of Clinical Chemistry and Pharmacology
• Publishing year: 1992
• Language: English
• Pages: 1157-1164
• Publication/Series: Neuropharmacology
• Volume: 31
• Issue: 11
• Links: http://dx.doi.org/10.1016/0028-3908(92)90012-E
• Document type: Journal article
• Publisher: Elsevier
• Topic: Pharmacology and Toxicology
• Keywords: NG 108-15 cell line; adaptation; inositol phosphates; bradykinin; G proteins; ethanol
• Status: Published
• Research group: Clinical Chemistry, Malmö
• ISBN/ISSN/Other: ISSN: 1873-7064