The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here: https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

Mass spectrometric monitoring of in vitro formed ad ducts between hexahydrophthalic anhydride (HHPA) and cysteine and lysine

På svenska

Author

Summary, in English

The in vitro formed adducts between hexahydrophthalic anhydride (HHPA) and cysteine and lysine were monitored by mass spectrometry. Freshly synthesized N-acetyl-S-hexahydrophthaloyl-L-cysteine was dissolved in dry acetonitrile and added to a solution of N-acetyl-L-lysine in 0.1 M sodium phosphate buffer (pH 7.4) an incubated at 37°C. The reactions were monitored by LC/MS on a triple quadrupole mass spectrometer with electrospray ionization coupled to a HPLC system from Perkin Elmer. The results show that there was a rapid rearrangement of HHPA from most of the cysteines to the lysines, and that HHPA-adducts of cysteine will be transferred to lysine when encountered.

Publishing year

2002

Language

English

Pages

325-326

Publication/Series

Proceedings 50th ASMS Conference on Mass Spectrmetry and Allied Topics

Document type

Conference paper

Publisher

American Society for Mass Spectrometry, Santa Fe, NM, 87505, United States

Topic

  • Environmental Health and Occupational Health

Keywords

  • Electrospray ionization (ESI)
  • Multiple reaction monitoring (MRM)
  • Hexahydrophthalic anhydride (HHPA)
  • Ion spray voltage

Conference name

Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Conference date

2002-06-02 - 2002-06-06

Conference place

Orlando, FL, United States

Status

Published