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Frequency determination of rare populations by flow cytometry: A hematopoietic stem cell perspective.

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Author

Summary, in English

Flow cytometry allows for identification of cellular subsets based on cell intrinsic properties, most often by the use of fluorochrome-conjugated antibodies recognizing distinct cell-surface epitopes that define the cells of interest. Advances in technical instrumentation and the availability of an ever-increasing number of fluorophores, today enables identification of multicolor defined cellular populations to a previously unreachable resolution. However, these possibilities put an increasing demand on preparation, acquisition, and subsequent analysis of the investigated samples. Identification of very rare cellular subsets, such as the bone marrow-residing hematopoietic stem cells (HSCs), causes further complexity to such analysis. Here, we discuss considerations and aspects in multicolor flow cytometry as exemplified by analysis of human and mouse HSCs. We illustrate advantages and drawbacks of polychromatic flow cytometry and propose strategies, such as the use of internal reference populations, for sample analysis. © 2013 International Society for Advancement of Cytometry.

Publishing year

2013

Language

English

Pages

721-727

Publication/Series

Cytometry Part A

Volume

83A

Issue

8

Document type

Journal article review

Publisher

John Wiley & Sons Inc.

Topic

  • Cell and Molecular Biology

Status

Published

Research group

  • Hematopoietic and immunologic developement

ISBN/ISSN/Other

  • ISSN: 1552-4930