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Gene therapy blockade of dorsal striatal p11 improves motor function and dyskinesia in parkinsonian mice.

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Author

  • Roberta Marongiu
  • Margarita Arango-Lievano
  • Veronica Francardo
  • Peter Morgenstern
  • Xiaoqun Zhang
  • Angela Cenci Nilsson
  • Per Svenningsson
  • Paul Greengard
  • Michael G Kaplitt

Summary, in English

Complications of dopamine replacement for Parkinson's disease (PD) can limit therapeutic options, leading to interest in identifying novel pathways that can be exploited to improve treatment. p11 (S100A10) is a cellular scaffold protein that binds to and potentiates the activity of various ion channels and neurotransmitter receptors. We have previously reported that p11 can influence ventral striatal function in models of depression and drug addiction, and thus we hypothesized that dorsal striatal p11 might mediate motor function and drug responses in parkinsonian mice. To focally inhibit p11 expression in the dorsal striatum, we injected an adeno-associated virus (AAV) vector producing a short hairpin RNA (AAV.sh.p11). This intervention reduced the impairment in motor function on forced tasks, such as rotarod and treadmill tests, caused by substantia nigra lesioning in mice. Measures of spontaneous movement and gait in an open-field test declined as expected in control lesioned mice, whereas AAV.sh.p11 mice remained at or near normal baseline. Mice with unilateral lesions were then challenged with l-dopa (levodopa) and various dopamine receptor agonists, and resulting rotational behaviors were significantly reduced after ipsilateral inhibition of dorsal striatal p11 expression. Finally, p11 knockdown in the dorsal striatum dramatically reduced l-dopa-induced abnormal involuntary movements compared with control mice. These data indicate that focal inhibition of p11 action in the dorsal striatum could be a promising PD therapeutic target to improve motor function while reducing l-dopa-induced dyskinesias.

Publishing year

2016-01-19

Language

English

Pages

1423-1428

Publication/Series

Proceedings of the National Academy of Sciences

Volume

113

Issue

5

Document type

Journal article

Publisher

National Academy of Sciences

Topic

  • Neurology

Status

Published

Research group

  • Basal Ganglia Pathophysiology

ISBN/ISSN/Other

  • ISSN: 1091-6490