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Staphylococcal Proteases Aid in Evasion of the Human Complement System.

På svenska

Author

  • Monika Jusko
  • Jan Potempa
  • Tomasz Kantyka
  • Ewa Bielecka
  • Halie K Miller
  • Magdalena Kalinska
  • Grzegorz Dubin
  • Peter Garred
  • Lindsey N Shaw
  • Anna Blom

Summary, in English

Staphylococcus aureus is an opportunistic pathogen that presents severe health care concerns due to the prevalence of multiple antibiotic-resistant strains. New treatment strategies are urgently needed, which requires an understanding of disease causation mechanisms. Complement is one of the first lines of defense against bacterial pathogens, and S. aureus expresses several specific complement inhibitors. The effect of extracellular proteases from this bacterium on complement, however, has been the subject of limited investigation, except for a recent report regarding cleavage of the C3 component by aureolysin (Aur). We demonstrate here that four major extracellular proteases of S. aureus are potent complement inhibitors. Incubation of human serum with the cysteine proteases staphopain A and staphopain B, the serine protease V8 and the metalloproteinase Aur resulted in a drastic decrease in the hemolytic activity of serum, whereas two staphylococcal serine proteases D and E, had no effect. These four proteases were found to inhibit all pathways of complement due to the efficient degradation of several crucial components. Furthermore, S. aureus mutants lacking proteolytic enzymes were found to be more efficiently killed in human blood. Taken together, the major proteases of S. aureus appear to be important for pathogen-mediated evasion of the human complement system. © 2013 S. Karger AG, Basel.

Publishing year

2014

Language

English

Pages

31-46

Publication/Series

Journal of Innate Immunity

Volume

6

Issue

1

Document type

Journal article

Publisher

Karger

Topic

  • Immunology in the medical area

Status

Published

Research group

  • Protein Chemistry, Malmö

ISBN/ISSN/Other

  • ISSN: 1662-811X